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Breeding scheme and house dust mite (HDM) exposure protocol. Female mice were exposed to HDM or vehicle (VEH) for 5 d/wk × 4 weeks before breeding, paired with male mice, and then exposed 5 d/wk until delivery. After reaching 8 weeks of age, offspring were sensitized to HDM or VEH on protocol Days 0 and 1 and then challenged with HDM on protocol Days 14–17. All experiments were conducted on protocol Day 18.
