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. 2022 Jul 12;122:930–935. doi: 10.1016/j.ijid.2022.07.022

Table 1.

Primers and probe sequences used for SARS-CoV-2 quantification.

Oligonucleotide Sequence (5’ à 3’) Length (bp) Ta (°C) Amplicon size (positions in reference genome)
Forward primer TCATCACGTAGTCGCAACAG 20 62.9 82 bp (28,832-28,913)
Reverse primer CATTGCCAGCCATTCTAGCA 20 63.9
Labeled Probea CAAGAAATTCAACTCCAGGCAGCAGTA 27 67.7
a

.- /56-FAM/CAAGAAATT/ZEN/CAACTCCAGGCAGCAGTA/3IABkFQ/ double-quenched probe.

bp: base pair.