Table 3.
Sample name | Dilution tested | CT obtained in RT-quantitative PCR |
Copies/µL | Final concentration (copies/mL) | ||||
---|---|---|---|---|---|---|---|---|
Replicates | Mean | SD | Replicates | Mean | SD | |||
Clinical sample 1 | 1/1000 | 24.0 | 23.7 | 0.3 | 407 | 496 | 92.7 | 5.0E+08 |
23.8 | 456 | |||||||
23.4 | 623 | |||||||
Clinical sample 2 | 1/100 | 23.5 | 23.4 | 0.1 | 923 | 971 | 58.4 | 9.7E+07 |
23.4 | 937 | |||||||
23.3 | 1,053 | |||||||
WHO international standard | 1/100 | 27.5 | 27.2 | 0.2 | 114 | 142 | 20.5 | 1.4E+07 |
27.0 | 163 | |||||||
27.1 | 1489 |
Ct: cycle threshold; PCR: polymerase chain reaction; WHO: World Health Organization.