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. 2022 Jul 11;12:11748. doi: 10.1038/s41598-022-15640-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene signature expression. (a) Genes of gene set differentially expressed in TNFα-treated, TGFß1-treated MEFs compared to control MEFs assessed by RT-qPCR. Fold changes are log2-transformed and expressed as a double gradient colormap. Log2 values of fold changes are censored at − 4 or 4 for better viewing. (b–c) Spearman correlations of genes expressed as log2(FC) assessed by RNA microarray (at 24 h) and by RT-qPCR (at 24 h) in TGFß1- (b) and TNFα-treated (c) MEFs compared to control MEFs. (d) Principal component analysis of MEFs (controls, treated by TGFß1 or TNFα) assessed by RT-qPCR. (e–g) Western Blot analysis of level of COL1A1 (e), αSMA (e), JAG1 (f), and DCN (g) in control, TGFß1- and TNFα-treated MEFs. Intensities of specific bands was measured by densitometry. ß-ACTIN was used for normalization of data. Experiment is representative of n = 3 independent experiments. Full unedited Western Blot are provided in supplementary Figs. S8–S10. (h) Quantification of CXCL1 (ng/ml) and MMP3 (ng/ml) in the supernatant sample (n = 12/group; 3 independent experiments). Numbers are expressed as median with IQR.