Figure 1.

Hesperetin inhibited cell proliferation. (A) The chemical structure of hesperetin. (B) GL261 cells were treated with hesperetin for 12, 24, and 48 h; cell viability was measured using the CCK-8 assay; and 1-way ANOVA was used in analyzing the results (n≥3). *, P<0.05 and ***, P<0.001 compared with the respective control group. (C) Cell morphology was observed after treating GL261 cells with hesperetin using a phase contrast microscope (0, 200, 400, and 800 µM) for 24 h. Scale bar represents 100 µm. TMZ, temozolomide; HSP, hesperetin. C represents control group (without HSP treatment). CCK-8, Cell Counting Kit-8 assay.