Figure 2.

| Spatiotemporal characterization of beta cell plateau activity. (A, F, J) Time course of the [Ca²⁺]_c oscillations, exposed to 8 mM glucose stimulation protocol with pH manipulations during the stable plateau phase. (B, G, K) Hexbin plot of all detected [Ca²⁺]_c oscillations presented as the time of the peak vs. the halfwidth of [Ca²⁺]_c oscillation duration. The sections in the color-coded rectangles were used for the further statistical analysis (orange=pH 7.1, green=pH 7.4 and purple=pH 7.7). (C, H, L) Quantification of frequency of the [Ca²⁺]_c oscillations expressed as number of events per minute per ROI. (D, I, M) Quantification of the [Ca²⁺]_c oscillations halfwidths. (E) In vitro insulin secretion from 50 isolated islets monitored over 75 min. Data is normalized to the basal insulin secretion at 6 mM glucose (5 min incubation), and presented as mean, the error bars represent standard deviation of the mean. Experiments from the individual islets are connected with a line. (**p < 0.01, ***p < 0.001).