Table 1.
Experimental setup overview.
| Sample # | Name | Analysis | Treatment | FBS starved |
|---|---|---|---|---|
| 1 | DMSO control 7 days | Proteomics/Phosphoproteomics | DMSO | N |
| 2 | DMSO control 7 days | Proteomics/Phosphoproteomics | DMSO | N |
| 3 | Palbo 7 days | Proteomics/Phosphoproteomics | PD0332991 1 μM | N |
| 4 | Palbo 7 days | Proteomics/Phosphoproteomics | PD0332991 1 μM | N |
| 5 | Palbo 1 day | Proteomics/Phosphoproteomics | PD0332991 1 μM | N |
| 6 | Palbo 1 day | Proteomics/Phosphoproteomics | PD0332991 1 μM | N |
| 7 | Palbo 7 days | Secretome | PD0332991 1 μM | Y |
| 8 | Palbo 7 days | Secretome | PD0332991 1 μM | Y |
| 9 | Palbo 7 days | Secretome | PD0332991 1 μM | Y |
| 10 | DMSO control 7 days | Secretome | DMSO | Y |
| 11 | DMSO control 7 days | Secretome | DMSO | Y |
| 12 | DMSO control 7 days | Secretome | DMSO | Y |
All samples collected and the analysis they were subjected to as indicated by the “Name” and “Treatment” columns. The “FBS starved” column indicates the replacement of standard growth medium containing foetal bovine serum (FBS) with FBS-free serum which is a standard procedure in secretome analysis. Y = yes. N= no.