Figure 1.

JunB is expressed in antigen-primed CD4⁺ T cells in vivo. 2 x 10⁶ naive OT-II cells (CD45.1⁺CD45.2⁺) were transferred to congenic recipient mice (CD45.1⁺), followed by immunization with OVA_323-339 peptides emulsified in CFA. At the indicated days post-immunization (dpi), cells were harvested from inaugural lymph nodes and analyzed. (A) Immunization scheme. i.v. intravenous injection, s.c. subcutaneous injection. (B) Flow cytometry analysis of JunB expression in transferred OT-II cells (CD3⁺CD4⁺CD45.1⁺CD45.2⁺, shown in red) and recipient naïve CD4⁺ T cells (CD3⁺CD4⁺CD45.1⁺, shown in blue) at the indicated dpi. Bar graphs show median fluorescence intensity (MFI) of JunB expression levels in CD44^hi OT-II cells and CD44^lo recipient cells. Error bars indicate s.d. (n =3). ****p < 0.0001, (unpaired two-tailed Student’s t-test).