Figure 3.

JunB is required for survival of CD4+ effector T cells in vivo. CFSE-labelled Junbfl/fl OT-II or Junbfl/flCd4cre OT-II cells (CD45.2+) were intravenously transferred to congenic recipient mice (CD45.1+), followed by immunization with OVA323-339 peptides emulsified in complete Freund’s adjuvant (CFA). At indicated days post-immunization (dpi), cells were harvested from inguinal lymph nodes and analyzed. Flow cytometry analysis of CFSE dilution (A), percentages of Zombie-NIR^-AnnexinV⁺ cells (B), and expression of Bim (C), as measured by median fluorescence intensity (MFI) (A–C) Error bars indicate s.d. (n = 4-6 wells per group). ns: non-significant, (p > 0.05) **p < 0.01, ****p < 0.0001, (unpaired two-tailed Student’s t-test). Data represent two independent experiments.