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. 2022 Jun 28;13:901030. doi: 10.3389/fimmu.2022.901030

Figure 4.

Figure 4

JunB is required for survival of TCR-stimulated CD4+ T cells. Naive CD4+ T cells isolated from Junbfl/fl or Junbfl/flCd4Cre mice were cultured in differentiation conditions for Th0, Th1, Th2, and Th17 cells and were analyzed by flow cytometry at indicated time points. (A) Zombie-NIR staining of cells cultured for 72 h. Numbers in histograms indicate average percentages of Zombie-NIR- cells. Bar graphs show percentages (top right) and absolute numbers (bottom right) of Zombie-NIR- cells. (B) Zombie-NIR and Annexin-V staining of cells cultured for 72 h. The bar graph (left) indicates percentages of Zombie-NIR- Annexin-V+ cells. (C) Analysis of Bim expression in cells cultured for 72 h. The bar graph indicates median fluorescence intensity (MFI) of Bim. (A–C) Error bars indicate s.d. (n = 4-6 wells per group). ns, non-significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, (unpaired two-tailed Student’s t-test). Data represent two independent experiments.