Figure 6.

JunB-dependent transcriptional regulation in T helper cell differentiation. Naive CD4⁺ T cells isolated from Junb^fl/fl or Junb^fl/flCd4^Cre mice were cultured under Th0-, Th1- and Th2-polarizing conditions for 48 h and subjected to RNA-seq analysis. Differentially expressed genes in Junb-deficient CD4⁺ T cells vs control cells (log₂ fold change > 0.5 or < -0.5, p value < 0.05, base mean > 100 TPM (normalized transcript per kilobase million)) were identified in each Th-polarizing condition. (A) Venn diagrams show the overlap of differentially expressed genes identified in Junb-deficient CD4⁺ T cells vs control cells under different Th-polarizing conditions. RNA-seq data for Junb-deficient CD4⁺ T cells and control cells cultured under Th17-polarizing conditions were from GSE 98414. (B) Scatter plots represent genes differentially expressed in Junb-deficient CD4⁺ T cells vs control cells under differentiation conditions for Th0, Th1, and Th2 cells. Genes considered significantly upregulated or downregulated are highlighted in red and blue, respectively. Genes with insignificant changes are highlighted in grey. On each plot, left top and right bottom numbers indicate numbers of upregulated or downregulated genes, respectively.