TABLE 1.

Primers and qPCR conditions of the genes studied (TA, Annealing Temperature; tE, Elongation time).

Gene	Amp. [bp]	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	TA [°C], tE [s]	Ref.
16S rDNA	160	CCTACGGGAGGCAGCAG	ATTACCGCGGCTGCTGGC	68, 20	Muyzer et al. (1993)
intl1	196	GCC​TTG​ATG​TTA​CCC​GAG​AG	GATCGGTCGAATGCGTGT	63, 20	Barraud et al. (2010)
sul1	163	CGC​ACC​GGA​AAC​ATC​GCT​GCA​C	TGA​AGT​TCC​GCC​GCA​AGG​CTC​G	68, 20	Pei et al. (2006)
sul2	722	CGG​CAT​CGT​CAA​CAT​AAC​C	GTGTGCGGATGAAGTCAG	65, 25	Maynard et al. (2003)
blaCMY-2	172	CGT​TAA​TCG​CAC​CAT​CAC​C	CGT​CTT​ACT​AAC​CGA​TCC​TAG​C	68, 20	Kurpiel and Hanson, (2011)
blaTEM	112	TTC​CTG​TTT​TTG​CTC​ACC​CAG	CTC​AAG​GAT​CTT​ACC​GCT​GTT​G	66, 20	Bibbal et al. (2007)
blaCTXM-32	155	CGT​CAC​GCT​GTT​GTT​AGG​AA	CGC​TCA​TCA​GCA​CGA​TAA​AG	63, 20	Stalder et al. (2014)
blaSHV	857	TCG​CCT​GTG​TAT​TAT​CTC​CC	CGC​AGA​TAA​ATC​ACC​ACA​ATG	55, 30	Maynard et al. (2003)
blaNDM-1	154	ATTAGCCGCTGCATTGAT	CAT​GTC​GAG​ATA​GGA​AGT​G	60, 20	Naas et al. (2011)
blaOXA-48	177	TGT​TTT​TGG​TGG​CAT​CGA​T	GTAAMRATGCTTGGTTCGC	55, 20	Monteiro et al. (2012)
blaVIM-2	382	GTT​TGG​TCG​CAT​ATC​GCA​AC	AATGCGCAGCACCAG GATAG	63, 20	Li et al. (2015)
vanA	1030	CAT​GAA​TAG​AAT​AAA​AGT​TGC​AAT​A	CCC​CTT​TAA​CGC​TAA​TAC​GAT​CAA	60, 35	Clark et al. (1993)
mcr-1	183	GGG​CCT​GCG​TAT​TTT​AAG​CG	CAT​AGG​CAT​TGC​TGT​GCG​TC	68, 20	Hembach et al. (2017)
mecA	91	CGC​AAC​GTT​CAA​TTT​AAT​TTT​GTT​AA	TGG​TCT​TTC​TGC​ATT​CCT​GGA	63, 20	Volkmann et al. (2004)