Figure S2.

Immunoblot of type I coronin depletion and rescue in COS-7 cells. (A) Representative immunoblots for type I coronin depletion (COR1A, COR1B, and COR1C) and rescue as in Fig. 3, B and D. Data show that type I coronins can be depleted efficiently and that the siRES COR1C constructs express well and at comparable levels for rescue (analysis was performed in triplicate). (B) Immunoblots for type I coronin depletion and rescue as in Fig. 5 showing relative expression of rescue constructs and endogenous COR1C. Representative immunoblots probed with antibody to COR1C, GFP, and GAPDH. (C) Quantification of rescue blots in B. Table shows average normalized ratios from three replicates (blots). Values were calculated as indicated in column headers. Briefly, the first column demonstrates clear KD. The second column demonstrates that expression is comparable between exogenously expressed mutants and is also comparable with endogenous. The third column demonstrates that the relative exogenous rescue expression is comparable to WT rescue. The final column is an estimate of CC expression relative to endogenous COR1C based on the average ratio of anti-GFP to anti-COR1C signal, suggesting that the CC is also not expressed above endogenous. This was necessary because the CC is not detectable via the anti-COR1C antibody and so could not be probed for in the same blot. Source data are available for this figure: SourceData FS2.