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. 2022 Jul 8;221(8):e202110089. doi: 10.1083/jcb.202110089

Figure S4.

Figure S4.

Vacuole diameter and bud length are not changed significantly in KD rescue experiments. (A) For each bud scored for fission in Fig. 5 C, vacuole diameter was also measured. Graph shows average vacuole diameter per cell, averaged per condition. No significant changes were observed in any condition, showing that only fission is affected and not other measures of endosome morphology. Data for graph from Halo E-vec: 139 endosomes in n = 15 cells; COR1C: 122 endosomes in n = 15 cells; COR1C ACT–: 213 endosomes in n = 17 cells; COR1C ΔCC: 281 endosomes in n = 18 cells; COR1C ACT– ΔCC: 296 endosomes n = 17 cells; and COR1C CC: 331 endosomes in n = 22 cells. X indicates mean, and line indicates median. Statistical analyses were performed with one-way ANOVA. (B) As in A, except FAM21-positive bud length was measured instead of vacuole diameter. Again, no significant changes were observed in any condition showing that only fission is affected and not other measures of endosome morphology. Data for graph from Halo E-vec: 139 endosomes in n = 15 cells; COR1C: 122 endosomes in n = 15 cells; COR1C ACT–: 213 endosomes in n = 17 cells; COR1C ΔCC: 281 endosomes in n = 18 cells; COR1C ACT– ΔCC: 296 endosomes n = 17 cells; and COR1C CC: 331 endosomes in n = 22 cells. X indicates mean, and line indicates median. Statistical analyses were performed with one-way ANOVA.