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Actin (green) LE (magenta) fission event from Fig. 1 G. COS-7 cell transfected with mCh-Rab7 (LE, magenta) and α-actin-mNG (actin, green) imaged every 2 s. Playback 2 frames/s. First arrow indicates marked bud pre-fission, following two sets of arrows indicate bud neck and departed bud. Note signal localizes only to bud neck and remains behind on the vacuole side.
