Skip to main content
. Author manuscript; available in PMC: 2022 Jul 12.
Published in final edited form as: Nat Protoc. 2021 Sep 17;16(11):5123–5145. doi: 10.1038/s41596-021-00605-2

Fig. 2 ∣. Mouse infusion workflow.

Fig. 2 ∣

An automated pump infuses the [13C] nutrient into the mice via a butterfly catheter inserted into the tail vein. At the end of infusion, the tumor is dissected and a sample is homogenized in 80% acetonitrile. The homogenized sample is dried and derivatized by MOX/TBDMS before injection onto GC-MS.