Figure 2.

Mouse immunization schedule and sample collection. C3H/HeJ mice were immunized with 25 μg of total antigen along with adjuvant (5 µg GLA–squalene–oil-in-water emulsion) on days 0, 21 and 42 respectively by intramuscular route. They were pre-bled prior to each immunization and prior to challenge infection and blood was obtained on day of necropsy. Control mice were immunized with PBS buffer plus adjuvant. Following immunization on day 52, mice were infected with live L. interrogans serovar Canicola (~1x10⁵ leptospires, LD₅₀ <100) by the intraperitoneal route. Blood and organs were collected after subsequent infection.* represent Adjuvant: GLA-SE, Glucopyranosyl lipid adjuvant–stable emulsion.