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CENPU promoted the proteasomal degradation of E2F6. (A-B) Immunoblotting analysis of E2F6 in Huh-7 and HCCLM3 cells after treatment with CHX for 0 h, 0.5 h, 1 h, and 2 h. (C-D) MG132 (10 µM) was applied to Huh-7 and HCCLM3 cells for 4 h and then immunoblotted for E2F6. (E) MG132 (20 µg/ml) was applied to HCC cells transfected with His-E2F6, HA-ubiquitin, CENPU plasmid, or si-CENPU, and 4 h later, total protein was extracted and subjected to co-IP using anti-His antibody.
