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. 2022 Jun 13;18(10):4026–4042. doi: 10.7150/ijbs.73493

Figure 3.

Figure 3

Effects of knocking down AKAP1 on renal function and mtDNA replication in the kidneys of diabetic mice. (A) Representative transmission electron microscopy images of podocyte foot processes and the glomerular basement membrane in control and diabetic mice. (B) Representative HE and PAS staining of glomeruli in the different groups. (Scale bars, 50 µm). (C) Representative Western blot and quantitative analysis of AKAP1, TFAM and BAX in the four groups. (n=3, *p < 0.05, ***p < 0.001). (D) Quantitative PCR analysis of AKAP1 and TFAM in the four groups. (n=3, *p < 0.05, ***p < 0.001). (E-F) Double fluorescent labeling of TFAM and synaptopodin and quantitative analysis in the glomeruli of the different groups. (Scale bars, 50 µm. n=6, ***p < 0.001). (G) Quantitative PCR analysis of mtDNA including ND1, cyt-b, COX I and ATPase6 in four groups. (n=3, *p < 0.05, ***p < 0.001). (H-I) DHE staining and quantitative analysis of the glomeruli in the four groups. (Scale bars, 50 µm. n=6, ***p < 0.001).