Table 2.
Reagents | Source | Identifier |
---|---|---|
Rabbit monoclonal anti-AKAP1 antibody | Cell Signaling Technology | Cat# 5203 |
Mouse monoclonal anti-Larp1 antibody | Santa Cruz Biotechnology | Cat# sc-515873 |
Rabbit polyclonal anti-Larp1 antibody | Proteintech | Cat# 13708-1-AP |
Mouse monoclonal anti-PKC antibody | Abcam | Cat# ab31 |
Mouse monoclonal anti-PKC antibody | Abcam | Cat# ab23511 |
Rabbit polyclonal anti-TFAM antibody | Proteintech | Cat# 22586-1-AP |
Rabbit polyclonal anti-Phospho-(Ser/Thr) Phe antibody | Cell Signaling Technology | Cat# 9631 |
Mouse monoclonal anti-BAX antibody | Proteintech | Cat# 60267-1-Ig |
Mouse monoclonal anti-TOM20 antibody | Santa Cruz Biotechnology | Cat# sc-17764 |
Mouse monoclonal anti-Synaptopodin antibody | Santa Cruz Biotechnology | Cat# sc-515842 |
Rabbit polyclonal anti-GAPDH antibody | GeneTex | Cat# GTX100118 |
HRP-Goat Anti-mouse IgG(H+L) | AntGene | ANT019 |
HRP-Goat Anti-rabbit IgG(H+L) | AntGene | ANT020 |
Alexa Fluor 488 Donkey anti Mouse IgG(H+L) | AntGene | ANT023S |
Alexa Fluor 594 Donkey anti Rabbit IgG(H+L) | AntGene | ANT030S |
Protein A+G agarose suspension beads | Calbiochem | Cat# IP05 |
Trizol reagent | Takara | T9108 |
cDNA synthesis kit | Thermo Fisher Scientific | Cat# K1622 |
SybrGreen qPCR mastermix | DBI Bioscience | DBI-2044 |
Dihydroethidium dye solution | Yeasen | 50102ES02 |
BeyoClick EdU kit with Alexa Fluor 488 | Beyotime | C0071S |
ROS assay kit | Beyotime | S0033M |
ATP assay kit | Beyotime | S0026 |
Mitochondrial membrane potential assay kit with JC-1 | Beyotime | C2006 |
MitoSOX red mitochondrial superoxide indicator | YEASEN | 40778ES50 |
Mitotracker Red CMXRos | YEASEN | 40741ES50 |
PE Annexin V apoptosis detection kit Enzastaurin |
BD Biosciences Targetmol |
559763 T6280-1 |
AKAP1 adenovirus | Vigene Biosciences | VH899251 |
AKAP1 siRNA | QIAGEN | Cat# 1027416 |
PKCβ siRNA | Santa Cruz Biotechnology | Cat# sc-29450 |
HiPerFect Transfection Reagent | QIAGEN | Cat# 301705 |
DNA Transfection Reagent | Roche | XTG9-RO |