Figure 2.

Mlkl deletion reduces leukocyte accumulation in the liver after CCl₄ treatment. (A) Schematic of the experimental design of CCl₄-induced acute or chronic liver damage in mice. (B) The amount of ALT and AST in the serum of WT and Mlkl^-/- mice treated with oil or CCl₄ (acute) (Oil groups, n=9; CCl₄ groups, n=12). (C) Quantification of the numbers of total leukcocytes, neutrophils, MoMFs, pro-inflammatory macrophages (pro-imms), anti-inflammatory macrophages (anti-imms), and Kupffer cells in the liver of WT and Mlkl^-/- mice treated with oil or CCl₄ (acute) (Oil groups, n=9; CCl₄ groups, n=12). (D) Serum level of CCL2, TNF-α, IFN-γ, and IL-1β in WT and Mlkl^-/- mice treated with oil or CCl₄ (acute) (Oil groups, n=9; CCl₄ groups, n=12). (E) Quantitative RT-PCR analysis of mRNAs encoding the chemokines: Ccl1, Ccl2, Ccl3, Ccl4, Ccl5, Cx3cl1, and the related chemokine receptors: Ccr1, Ccr2, Cxcr3, Cx3cr1 in the whole liver samples from WT or Mlkl^-/- mice treated with oil or CCl₄ (acute). (Oil groups, n=9; CCl₄ groups, n=12). (F) The amounts of ALT and AST in the serum of the WT and Mlkl^-/- mice treated with oil or CCl₄ (chronic) (Oil groups, n=10; CCl₄ groups: WT, n=22; KO, n=20). (G) Quantification of the numbers of total leukocytes, neutrophils, MoMFs, pro-imms, anti-imms, and Kupffer cells in the liver of WT and Mlkl^-/- mice treated with oil or CCl₄ (chronic) (Oil groups, n=10; CCl₄ groups: WT, n=22; KO, n=20). Data are shown as Means ± SEM, *P < 0.05, **P< 0.01, ***P< 0.001 (Student's t-test).