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. 2022 Jun 28;8(7):1303–1315. doi: 10.1021/acsinfecdis.2c00075

Figure 3.

Figure 3

Application of PI 16:0_19:0 as a correlate of Mtb load in model systems. (A) Detection of Mtb in murine BMDM. MS detection level either on basis of accurate mass determination in MS1 (m/z 851.5655) or MS2 (FA 19:0, m/z 851.5 → 297.2799; Figure S4A) (B) validation of the Mtb infection status for neutrophils isolated from five healthy donors. At 6 hpi, CFUs (left axis) and amount of PI 16:0_19:0 (right axis) were determined. Data from different donors are indicated by distinctive symbols with double determination for each individual. In noninfected cultures, PI 16:0_19:0 was below the limit of detection. (C) Pulmonary mycobacterial burden in IL-13WT and IL-13TG mice upon Mtb aerosol infection by CFU analysis at indicated time points and (D) respective concentrations of PI 16:0_19:0 determined from the same lung tissue homogenates. The dotted line indicates basal concentration in uninfected mice (1.75 × 10–2 pmol/μg; n = 11, data file S2). One-way ANOVA analysis was performed for data presented in panels (C,D).