Figure 10.
Compound 16 displays potent cell growth inhibition and EZH2 degradation in primary TNBC patient cells (515a ductal TNBC). (A) 515a cells were treated with serial dilution of 16 (starting from 10 μM, with 2-fold dilution) for 5 days. Cell viability was determined using a WST-8 assay. GI50 results shown are the mean values ± SD from three independent experiments. (B) 515a cells were treated with DMSO and 16 at concentrations of 0.1, 0.5, and 1 μM for 48 h, and the cell lysate was analyzed with Western blotting to assess the EZH2 protein level. β-Actin was used as the loading control. Results shown are representative of two independent experiments.