Figure 6.

Compound 16 induces EZH2 degradation in a time- and concentration-dependent manner. (A) Western blots of EZH2, SUZ12, EED, and H3K27me3 post-treatment of MDA-MB-453 cells with DMSO (48 h) or compound 16 (1 μM) at the indicated time points. β-Actin and H3 were used as the loading controls. (B) Quantification of EZH2 blots in (A) and their replicates. (C) Western blots of EZH2, SUZ12, and EED post-treatment of MDA-MB-453 cells with DMSO or compound 16 at the indicated concentrations for 48 h. β-Actin was used as the loading control. (D) Quantification of EZH2 blots in (C) and their replicate. (E) DC₅₀ curve of 16, generated using the data in (D).