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Measuring extracellular acidification rates using glycolytic rate assay revealed that the aged BMVs has compromised basal glycolysis (a), compensatory glycolysis (b), and basal proton efflux rate (PER) (c). % PER from glycolysis (d), glycoPER/Mito OCR (e) and post 2-DG acidification (f) were unaltered between the young and aged BMVs. Data were represented as mean ± SD and analyzed by student’s t-test. P ≤ 0.05 was taken as the statistical significance. N = 7–9 mice/age group.
