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Measuring oxygen consumption rate (OCR) using Mito stress assay revealed that aged BMVs have impaired mitochondrial respiration and citrate synthase activity. (a) Basal Respiration. (b) ATP production. (c) Maximal respiration. (d) Spare capacity. (e) Proton leak. (f) Non-mitochondrial respiration. (g). Citrate synthase activity. Data were represented as mean ± SD and analyzed by student’s t-test. P ≤ 0.05 was taken as the statistical significance. N = 8–12 mice/age group.
