Figure 6.

Measuring OCR using Mito fuel assay revealed that mitochondria from the aged BMVs utilize more glutamate for oxidative phosphorylation. (a). % of utilization of metabolic fuels by cerebral microvascular mitochondria. (b). Glutamate dehydrogenase (GDH) activity. (c). Representative western blots and bar graph for carnitine palmitoyltransferase 1a (CPT1a) and β-actin proteins and their density ratio. Data were represented as mean ± SD and analyzed by student’s t-test. P ≤ 0.05 was taken as the statistical significance. N = 4 (pooled from 12 mice) for young mice and N = 3 (pooled from 9 mice) for aged mice. N = 11–12 mice for GDH activity, and N = 10 mice for CPT1a westerns. All the original immunoblots are included in the online supplementary figures (15–16).