Table 1.

Performance of mNGS for genotypic prediction of antimicrobial susceptibility compared to a reference standard of clinical microbiologic testing. Sensitivity, specificity, and accuracy of DNA + RNA mNGS compared to a reference standard of clinical antimicrobial susceptibility testing based on Clinical & Laboratory Standards Institute (CLSI) minimum inhibitory concentration (MIC) breakpoints. A Gram-positive pathogens. B Gram-negative pathogens. AMR gene(s) detected by mNGS indicated. With respect to genotype-phenotype predictions, squares filled red indicate true positives, squares filled blue indicate true-negatives, squares with purple text = false negatives, squares with orange text are false positives

*mutations in PBP1a/2x, Sens Sensitivity, Spec Specificity, TN True negative, FN False negative; n/a phenotypic susceptibility to antibiotic not tested in the clinical laboratory. 95% confidence interval (CI) listed below each sensitivity and specificity value