FIG. 1.

Complementation of cadium-sensitive mutant 06909s22x (cadA::km). (A) Map of a 4.2-kb PstI fragment carrying cadA and cadR. (B) Complementation of cadmium sensitivity by different subclones. P_lac indicates lac promoter in pRK415. Restriction endonuclease sites of the subclones are not indicated. A “+” indicates complementation of cadmium sensitivity to cadmium resistance, but a “−” indicates no complementation. Abbreviations: C, ClaI; E, EcoRI; H, HindIII; K, KpnI; P, PstI; Sp, SphI; Xh, XhoI.