FIG. 4.

Southern blot hybridization of the cadA gene of P. putida 06909 to total DNA from cadmium-resistant Pseudomonas species and other gram-negative bacteria. The MICs (mM) of cadmium chloride for each strain are indicated in parentheses with the original strain reference in brackets as follows: lane 1, P. putida 06909 (1.7 [59]); lane 2, P. putida 08891 (4.0 [9]); lane 3, P. fluorescens 09906 (0.7 [58]); lane 4, P. fluorescens 2-79 (0.7 [55]); lane 5, P. fluorescens 08908 (>4.0 [8]); lane 6, P. fluorescens 0785-17 (1.0 [30]); lane 7, P. fluorescens 08892 (0.1 [9]); lane 8, P. fluorescens 513 (0.1); lane 9, P. aeruginosa PAO1 (4.0 [17]); lane 10, P. stutzeri ATCC 17588 (4.0); lane 11, P. cichorii 07881 (0.1 [9]); lane 12, P. syringae pv. syringae PS61 (0.5 [3]); lane 13, P. syringae pv. tomato (0.4 [2]); lane 14, Pseudomonas sp. strain 02894 (2.0 [8]); lane 15, Pseudomonas sp. strain 07887 (1.0 [8]); lane 16, Pseudomonas sp. strain 07888 (1.2 [8]); lane 17, Xanthomonas axonopodis pv. vesicatoria 07882 (0.2 [9]); lane 18, Agrobacterium radiobacter K84 (0.05 [34]); lane 19, E. coli DH5α (0.2 [44]); and lane 20, plasmid DNA of pUIVS22 carrying the cadA gene. The DNA in each lane was completely digested with BamHI, and a total of 2 μg of DNA per lane was loaded for each sample. A total of 100 ng of plasmid DNA was loaded for the plasmid in the lane 20.