Alignment of the L. lactis IO-1,
L. lactis NRRL B-4449, and L. lactis
210 XylM sequences with seven galactose mutarotases. GenBank accession
numbers for the GalM sequences are as follows: A.
calcoaceticus, X03893; S. thermophilus, M38175;
E. coli, U13636; H. influenzae, X65934;
L. lactis ATCC 7962, U60828; L.
lactis MG1363, AJ011653; and Lactobacillus
casei,
AF005933. Three consensus sequences are indicated above
the alignment. Consensus sequence 1 includes residues that are
conserved in all 10 mutarotases. Consensus sequence 2 includes residues
that are conserved in 8 of 10 mutarotases. Consensus sequence 3
includes residues that are similar in 8 of 10 mutarotases. Residue
groupings of consensus sequence 3 are as follows: a, acidic residues
(DE); b, basic (HKR); f, aliphatic (AGILV); m, amide (NQ); o, aromatic
(FWY); h, hydroxyl (ST); i, imino (P); and s, sulfur (CM). Residues
identical to those in IO-1 XylM are boxed.