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. 2001 Apr;67(4):1445–1452. doi: 10.1128/AEM.67.4.1445-1452.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 3 — SDS-PAGE analysis of L. lactis recombinant mutarotase-His tag fusion protein purified from E. coli BL21 using nickel column chromatography. Lane 1, molecular weight markers (low series; Bio-Rad); lane 2, nickel column eluate from recombinant E. coli BL21 carrying L. lactis 210 xylM; lane 3, flowthrough of nickel column eluate from recombinant E. coli BL21 carrying L. lactis 210 xylM; lane 4, nickel column eluate from recombinant E. coli BL21 carrying L. lactis NRRL B-4449 xylM; lane 5, flowthrough of nickel column eluate from recombinant E. coli BL21 carrying L. lactis NRRL B-4449 xylM; lane 6, nickel column eluate from recombinant E. coli BL21 carrying L. lactis IO-1 xylM; lane 7, flowthrough of nickel column eluate from recombinant E. coli BL21 carrying L. lactis IO-1 xylM.