(Top) Autoradiograph of RNA isolated from xylose-grown
and glucose-grown L. lactis IO-1, L.
lactis 210, and L. lactis NRRL B-4449 probed
with a region including xylB and xylM.
Lanes 1, 4, 7, and 10, L. lactis 210; lanes 2, 5, 8, and
11, L. lactis NRRL B-4449; lanes 3, 6, 9, and 12,
L. lactis IO-1. (Bottom) Transcription analysis of the
xyl and xyn loci of xylose-grown
L. lactis IO-1, L. lactis 210, and
L. lactis NRRL B-4449. The top portion of panel B
shows the organization and sizes of the genes (indicated by horizontal
arrows) and intergenic regions (vertical lines ending in open
triangles) and the locations of the Northern probes (rectangles). The
bottom portion of panel B shows the predicted transcript boundaries
(solid lines or, where there is uncertainty, broken lines). The
transcripts (sizes indicated on the right) which hybridized to the
probes (listed on the left) could lie anywhere within the depicted
boundaries.