TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genetic marker(s) | Source |
|---|---|---|
| Strains | ||
| Lactococcus lactis subsp. lactis 210 | Xyl− | J. Kondo (Marschall Products) |
| Lactococcus lactis subsp. lactis IO-1 | Xyl+ | P. Stansbury (University of Hertfordshire, Hertfordshire, United Kingdom) |
| Lactococcus lactis subsp. lactis NRRL B-4449 (formerly Lactobacillus xylosus) | Xyl+ | A. J. Sinskey (Massachusetts Institute of Technology) |
| Escherichia coli DH5α F′ | F′ endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacIZYA-argF) U169 deoR [φ80dlacΔ(lacZ)M15] | |
| Escherichia coli BL21 (DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3 [T7lac promoter]) | Novagen |
| Plasmids | ||
| pGEM-T | AprlacZ′ | Promega |
| pET19d | Apr | Novagen |