Figure 5.

Imbalanced depolarization-hyperpolarization in HS cells within a stride correlates with rapid steering adjustments

(A) Schematic with time windows used for analysis.

(B) ΔVm (left), or its temporal derivative dVm/dt (right), Va, and Vf triggered at the local Vf peak in walking segments drifting leftwards (Va < −50°/s, grand mean ± SEM, n = 19 fly cell pairs). Traces were separated based on drift adjustment after the peak in Vf. Arrowheads highlights an overall increase in ΔVm for segments with rapid adjustments (black trace).

(C) dVm/dt as a function of the stride cycle of the left front leg during low (−50 < Va < 0°/s) or high (−200 < Va < −150°/s) drift.

(D) Depolarization-hyperpolarization balance across strides during low or high drift (p = 0.00016, Z = −3.78, n = 19 fly cell pairs, signed-rank test).

(E) Mean dVm/dt before peak Vf (−200:0 ms window, with peak Vf at 0, see A) as a function of the mean drift attenuation after peak Vf (0–200-ms window) for every segment (n = 1,378 segments from 19 fly cell pairs). The linear regression fit is indicated.

(F) Correlation between dVm/dt before peak Vf and drift attenuation after Vf per cell. The correlation was consistently positive (p = 0.00013, Z = 3.82, n = 19 fly cell pairs, the signed-rank test).

See also Figure S6.