REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-nc82	DSHB	Cat # nc82; RRID:AB_2314866
Rabbit polyclonal anti-GFP	Thermo Fisher Scientific	Cat # A6455; RRID: AB_221570
Alexa Fluor 633 conjugated goat anti-mouse	Thermo Fisher Scientific	Cat # A21050; RRID: AB_2535718
Alexa Fluor 488 conjugated goat anti-rabbit	Thermo Fisher Scientific	Cat # A11008; RRID: AB_143165
Rabbit monoclonal anti-HA	Cell Signaling Technologies	Cat # 3724S; RRID:AB_1549585
Rat monoclonal anti-FLAG	Novus Biologicals	Cat # NBP1-06712; RRID:AB_1625981
Alexa Fluor 594 conjugated goat anti-rabbit	Thermo Fisher Scientific	Cat # A-11012; RRID:AB_2534079
Alexa Fluor 647 conjugated goat anti-rat	Thermo Fisher Scientific	Cat # A-21247; RRID:AB_141778
Alexa Fluor 488 conjugated goat anti-mouse	Thermo Fisher Scientific	Cat # A28175; RRID:AB_2536161
Mouse monoclonal anti V5-tag:DyLight®550	Bio-Rad	Cat # MCA1360D550GA; RRID:AB_2687576
Chemicals, peptides, and recombinant proteins
Histamine	Sigma	Cat # H7125
All trans-retinal	Sigma	Cat # R2500
Collagenase IV	Worthington	Cat # CLS-4
Biocytin hydrazide	Thermo Fisher Scientific	Cat # B1603
Alexa Fluor 568 Hydrazide	Thermo Fisher Scientific	Cat # A10437
PFA	Electron Microscopy Sciences	Cat # 15710
Normal mouse serum	Jackson Immuno Research Laboratories	Cat # 015-000-120
Normal goat serum	Life Technologies	Cat # PCN5000
Vectashield	Vector Laboratories	Cat # H-1200
Deposited data
Custom MATLAB codes	This paper	https://github.com/ChiappeLab/Analysis-Walking-Stride-Modulation
Experimental models: Organisms/strains
Drosophila melanogaster: w+; UAS-Ort; +	Rister et al., 2007	N/A
Drosophila melanogaster: w∗; UAS-2xEGFP; +	Bloomington Drosophila Stock Center (BDSC)	RRID: BDSC_6874
Drosophila melanogaster: w1118; +; VT058487-GAL4	Vienna Drosophila Resource Center (VDRC)	N/A
Drosophila melanogaster: wDL	Tammero and Dickinson, 2002	N/A
Drosophila melanogaster: w1118; +; R81G07-GAL4	BDSC	RRID: BDSC_40122
Drosophila melanogaster: w∗, NorpA[36]	BDSC	RRID: BDSC_9048
Drosophila melanogaster: w1118; +; 20XUAS-IVS-CsChrimson.mVenus	BDSC	RRID: BDSC_55136
Drosophila melanogaster: w1118; R39E01-lexA; +	BDSC	RRID: BDSC_52776
Drosophila melanogaster: y1 w∗; 13XLexAop2-6XGFP(VK00018)/CyO; Dr1/TM6C, Sb1 Tb1	BDSC	RRID: BDSC_52265
Drosophila melanogaster: y1 w∗; wgSp-1/CyO; 20XUAS-6XGFP(attp2)	BDSC	RRID: BDSC_52262
Drosophila melanogaster: w1118; R11H10-p65.AD(attp40); VT025925-ZpGal4DBD(attp2)	Bidaye et al., 2020 and Salil Bidaye	N/A
Drosophila melanogaster: w, 5-40-GAL4; dacREFLP;+	Giorgianni and Mann, 2011; Hughes and Thomas, 2007 and César S Mendes	N/A
Drosophila melanogaster: w∗; UAS-FRT-stop-FRT-TNT/Cyo; +	BDSC	RRID: BDSC_67690
Drosophila melanogaster: w1118; R27B03-p65.AD(attp40); +	BDSC	RRID: BDSC_70164
Drosophila melanogaster: w1118; +; VT058488-GAL4.DBD(attp2)	BDSC	RRID: BDSC_72871
Drosophila melanogaster: w1118; p65-AD.empty(attp40); GAL4-DBD.empty(attp2)	BDSC	RRID: BDSC_79603
Drosophila melanogaster: w∗; +; UAS-ReaChR(VK00005)	BDSC	RRID: BDSC_53749
Drosophila melanogaster: w1118; R46A02-p65.AD(attp40); +	BDSC	RRID: BDSC_71064
Drosophila melanogaster: w1118; R34E09-p65.AD(attp40); VT023823-GAL4.DBD(attp2)/TM6B	BDSC	RRID: BDSC_86632
Drosophila melanogaster: wDL; wDL; 10XUAS-IVS-eGFPKir2.1(attP2)	von Reyn et al., 2014 and Gwyneth Card	N/A
Drosophila melanogaster: w1118; UAS-sytGCaMP6s(attp40)/Cyo; TM2/TM6B	Cohn et al., 2015 and Vanessa Ruta	N/A
Drosophila melanogaster: w1118; +; UAS-CD4-Tdtomato(VK00033)	BDSC	RRID: BDSC_35837
Drosophila melanogaster: w1118, hs-FLPG5.PEST; +; 10xUAS(FRT.stop)myr::smGdP-HA (VK00005), 10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdP-FLAG(su(Hw)attP1)	BDSC	RRID: BDSC_64085
Software and algorithms
MATLAB	The Mathworks	https://www.mathworks.com
JAABA	Kabra et al., 2013	http://jaaba.sourceforge.net
DeepLabCut	Mathis et al., 2018	https://github.com/DeepLabCut
Ephus	Suter et al., 2010	http://scanimage.vidriotechnologies.com/display/ephus/Ephus
ScanImage	Pologruto et al., 2003	http://scanimage.vidriotechnologies.com/display/ephus/ScanImage
NeuPrint	Scheffer et al., 2020	https://neuprint.janelia.org
Computational Morphology Toolkit (CMTK)	Rohlfing and Maurer, 2003	https://www.nitrc.org/projects/cmtk/
Natverse	Bates et al., 2020	https://natverse.org
Color-Depth MIP	Otsuna et al., 2018	https://www.janelia.org/open-science/color-depth-mip
DeepEthogram	Bohnslav et al., 2021	https://github.com/jbohnslav/deepethogram