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Molecular mass determination of the native dye-l-proDH. The molecular mass was determined on the native PAGE using a 2 to 15% gradient gel as described in the text. Cycloglobulin (669 kDa), ferritin (443 kDa), lactate dehydrogenase (140 kDa), bovine serum albumin (66 kDa), and trypsin inhibitor (20 kDa) were used as the molecular mass standards (Daiichi Chemical, Tokyo, Japan).
