TABLE 1.
Step | Total activity (U) | Total protein (mg) | Sp act (U/mg) | Yield (%) |
---|---|---|---|---|
Crude extract | 23.0 | 816 | 0.028 | 100 |
Red-Sepharose-CL4B | 21.4 | 185 | 0.116 | 93 |
Butyl-Toyopearl | 12.2 | 9.18 | 1.34 | 53 |
Uno Q | 5.93 | 0.945 | 6.28 | 26 |
Superdex 200 | 2.34 | 0.760 | 3.08 | 10 |