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. 2022 Jun 17;18(6):e1010507. doi: 10.1371/journal.ppat.1010507

Fig 3. Alterations in cellular trafficking induced by ΔGY and in vivo acquired mutations.

Fig 3

(A) Partial sequences for SIVmac239 Env and human CD4 are shown with the membrane spanning domain (MSD, shaded) and CD (partial for SIVmac239 Env and full length for CD4). A CD4-SIV Env CD construct is shown containing the CD4 ecto- and MSD with 7 a.a. from the membrane proximal cytoplasmic domain of CD4 (underlined) fused to a.a. 716–745 from the SIVmac239 Env CD. CD4-SIV Env CD chimeras are shown containing ΔGY and indicated changes (bold). (B) Steady state distribution of the CD4-SIV Env CD chimeras in stably transformed HeLa cells analysed by immunofluorescence microscopy using an anti-CD4 antibody. (C) Cellular distribution of CD4-SIV Env CD chimeras following incubation of cells with anti-CD4 antibody (Q4120; 3 hr at 37°C) prior to fixation. Confocal Z stacks were deconvolved and displayed as maximum projections. Size bars = 10 μm.