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. 2022 Jun 17;18(6):e1010507. doi: 10.1371/journal.ppat.1010507

Fig 5. ΔGY and in vivo acquired mutations modulate Env sorting in polarized epithelial cells.

Fig 5

(A) Cell surface distribution of CD4-SIV Env truncated CD chimeras containing the indicated Env CDs from Fig 3 on polarized MDCKII cells. Apical, basal, and lateral surfaces are indicated. (B) Cell surface distribution of CD4-SIV Env chimeras containing a full-length SIVmac239 CD. (C) Cell surface distribution of native, full length SIVmac239 Env with or without the ΔGY mutation. Left panels show orthogonal deconvolved immunofluorescence projections of representative cells; right panels show quantitation of the images as a ratio of the basal + lateral to apical labelling intensity calculated as described in the Material and Methods. Data shown are the means of measurements from 58–223 cells per condition imaged from (A) n≥3 (except ΔGY + ΔQTH [AFQL] which is n = 1) (B) n≥2 and (C) n = 2 independent experiments. Scale bar = 5 μm.