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. 2022 Jun 17;18(6):e1010507. doi: 10.1371/journal.ppat.1010507

Fig 8. The IRL set restores polarized sorting of Env but not endocytosis.

Fig 8

(A) The C-terminal sequences of CD4-SIV Env CD constructs (Fig 3A) with sequences from the CD4 CD underlined. Constructs contained the CD from WT SIVmac239 or SIVmac239ΔGY+R722G+IRL (red). (B) Endocytosis of CD4-SIV Env truncated CD constructs in HeLa cells. Left panel shows the % of cell surface CD4 at the indicted times (0 mins = 100%); right panel shows the rate of CD4 endocytosis over the first 5 mins after warm-up. Graphs display the mean ± SEM (left panel) or the values from 3 independent experiments (right panel). (C) Cell surface CD4-SIV Env CD constructs on polarized MDCKII cells. Left panels show orthogonal deconvolved immunofluorescence projections of representative cells; right panel shows quantitation of the ratio of basolateral to apical signal from the left panels. The data show the mean of between 59–190 cells per condition and the values from n≥2 independent experiments. Scale bar = 5 μm.