Figure 3.

Omentin-1 promoted extracellular matrix synthesis in HNPCs. HNPCs were stimulated with IL-1β (10 ng/mL), with or without Omentin-1 (150,300 ng/ml) for 24 h. a. Cell cycle was analyzed by flow cytometry (n = 3/group). b. Cell viability of HNPCs from each group was assessed by CCK8 assay (n = 3/group). c. Colleagen II, Aggrecan, MMP 13 and ADAMT5 mRNA expression levels (n = 3/group). d, e. Colleagen II, Aggrecan, MMP 13 and ADAMT5 protein expression levels (n = 3/group). *P < 0.05 between control and IL-1β groups, ^#P < 0.05 between IL-1β and IL-1β+Omentin-1 (150 ng/ml) groups, ⁺P < 0.05 between IL-1β+Omentin-1 (150 ng/ml) and IL-1β+Omentin-1 (300 ng/ml) groups.