TABLE 1.

PCR and sequencing primers used in this study^a

Primer	Sequence (5′ to 3′)
28F*	AAG AGT TTG ATC CTG GCT CAG A
525F	AAC TCC GTG CCA GCA GC
1492R*	TAC GGC TAC CTT GTT ACG ACT T
M13 reverse	CAG GAA ACA GCT ATG AC
M13 (−20) forward	GTA AAA CGA CGG CCA GT

^aPrimer abbreviations: F, forward; R, reverse. Numbering of 16S rDNA-primers is based on the E. coli 16S rRNA gene (5). An asterisk indicates that these primers correspond to fD1 and rP2 (7), with the exceptions shown in bold letters.