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. 2022 May 12;13(5):11923–11932. doi: 10.1080/21655979.2022.2065952

Figure 2.

Figure 2.

KIAA1429 accelerated the aerobic glycolysis and malignant phenotype of CRC cells. (a) KIAA1429 expression was enhanced using plasmids transfection in SW480 cells, and KIAA1429 expression was also silenced using shRNA transfection in HT29 cells. The transfection efficient was detected using RT-PCR. (b) The transfection efficient was detected using western blot. (c) Glucose uptake analysis was performed to reveal the glucose uptake quantitation in SW480 cells with KIAA1429 overexpression, and in HT29 cells with KIAA1429 silencing. (d) Lactate analysis was detected to reveal the lactate production in SW480 cells with KIAA1429 overexpression, and in HT29 cells with KIAA1429 silencing. (e) ATP analysis was performed to illustrated the ATP production in SW480 cells with KIAA1429 overexpression, and in HT29 cells with KIAA1429 silencing. (f, g) Extracellular acidification rate (ECAR) analysis reveals the glycolysis rate. *p < 0.05; **p < 0.01.