Figure 3.
MKLN1-AS knockdown enhances the inhibitory effects of lenvatinib in HCC cells.
(a) The IC50 values of lenvatinib in HuH-7 and SK-Hep-1 at 48 h were assessed by CCK-8 assay. (b) The histogram represents the percentage of cells found in the living (Annexin V− & PI−), early apoptotic (Annexin V+ & PI−), late apoptotic (Annexin V+ & PI+), and necrotic (Annexin V− & PI+) populations from SK-Hep-1 and HuH-7 treated with lenvatinib. The apoptosis of HCC cells was measured using the flow cytometry assay.