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. 2022 May 4;13(5):11417–11429. doi: 10.1080/21655979.2022.2063222

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Effects of HMGCS2 overexpression on cell proliferation and apoptosis of cardiomyocytes. (a-c) The expression of HMGCS2(a), ANP(b) and BNP(c) mRNA in cardiomyocytes with pcDNA3.1 or pcDNA3.1-HMGCS2 transfection was presented. (d) Cardiomyocyte viability was measured by CCK-8 assay at 72 h post-treated with pcDNA3.1 or pcDNA3.1-HMGCS2. (e) Cardiomyocyte apoptosis was measured by TUNEL assay at 24 h post-transfected with pcDNA3.1 or pcDNA3.1-HMGCS2. (f and g) Cardiomyocyte caspase-3 and caspase-9 activities were evaluated by caspase-3 and caspase-9 activity kits at 24 h post-transfected with pcDNA3.1 or pcDNA3.1-HMGCS2. N = 3. Significant level was annotated as *P < 0.05 and **P < 0.01.