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. 2022 Jun 15;13(5):13815–13828. doi: 10.1080/21655979.2022.2084460

Figure 6.

Figure 6.

Effects of miR-1225-3p on the survival and apoptosis of KFs via AFF4. (a) The transfection efficiency of AFF4 mRNA was evaluated by qRT-PCR in KFs transfected with si-AFF4 or miR-1225-3p inhibitor. (b) The AFF4 protein level was evaluated by western blot in KFs transfected with si-AFF4 or miR-1225-3p inhibitor. (c) CCK-8 assays were performed to assess the viability in KFs transfected with si-AFF4 or miR-1225-3p inhibitor. (d) BrdU assays were performed to assess the proliferation ability in KFs transfected with si-AFF4 or miR-1225-3p inhibitor. (e) Cell apoptosis was examined using flow cytometry in KFs transfected with si-AFF4 or miR-1225-3p inhibitor. (f) Transwell migration assays were applied for assessing the migration ability of KFs transfected with si-AFF4 or miR-1225-3p inhibitor. *P < 0.05, **P < 0.001 vs. si-NC; ##P < 0.001 vs. si-AFF4+ inhibitor-NC.