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. 2022 Jun 19;13(5):13970–13985. doi: 10.1080/21655979.2022.2084235

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DDX56 is associated with GC cell proliferation. (a, c) EdU assays and quantitative results for AGS cells after transfection with plasmid. (b, d) EdU assays and quantitative measurements for MKN28 cells after transfection with plasmid. (e) Proliferation of plasmid-transfected AGS cells was measured by CCK8 assay. (f) Cell viability of plasmid-transfected MKN28 cells was determined using CCK8 assay. (g-j) Representative images and quantitative flow cytometry results for cell cycle arrest in AGS and MKN28 cells transfected with NC-DDX56 plasmid and sh-DDX56-2 plasmid. Abbreviations: CCK-8, Cell Counting Kit-8. Asterisks indicate statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, P > 0.05, not significant.