Figure 4.

GADD45A silencing promotes proliferation and invasiveness of trophoblast cell lines possibly through suppressing the MAPK signaling pathway. A, GADD45A mRNA in HTR-8/SVneo and JEG3 cells after transfection of sh-NC or sh-GADD45A determined by qPCR analysis; B, viability of cells examined by the MTT assay; C, proliferation activity cells examined by the EdU labeling assay; D, migration ability of HTR-8/SVneo and JEG3 cells examined by the scratch test; E, invasiveness of HTR-8/SVneo and JEG3 cells examined by the transwell assay; F-H, GADD45A enriched signaling pathways analyzed by the KEGG enrichment analysis; I, protein level and phosphorylation of p38 in cells examined by WB analysis. Repetition = 3. *p < 0.05; **p < 0.01.