Figure 5.

Silencing of GADD45A restores proliferation and invasiveness of trophoblast cell lines suppressed by sh-EZH2. A, EZH2 and GADD45A mRNA in HTR-8/SVneo cells after transfection of sh-EZH2 alone or sh-EZH2 + sh-GADD45A determined by qPCR analysis; B, viability of cells determined by the MTT assay; C, proliferation activity of cells measured by the EdU labeling assay; D, migration of HTR-8/SVneo and JEG3 cells examined by the scratch test; E, invasiveness of HTR-8/SVneo and JEG3 cells examined by the transwell assay; F, protein level and phosphorylation of p38 in cells determined by WB analysis. Repetition = 3. *p < 0.05; **p < 0.01.